![PBST [10X]; Phosphate buffered saline with Tween-20; 80mM Na2HPO4, 1.5M NaCl, 20mM KH2PO4, 30mM KCl, 0.5% Tween-20, pH 7.4 - Cepham Life Sciences Research Products PBST [10X]; Phosphate buffered saline with Tween-20; 80mM Na2HPO4, 1.5M NaCl, 20mM KH2PO4, 30mM KCl, 0.5% Tween-20, pH 7.4 - Cepham Life Sciences Research Products](https://www.cephamls.com/wp-content/uploads/2019/02/10385-1.jpg)
PBST [10X]; Phosphate buffered saline with Tween-20; 80mM Na2HPO4, 1.5M NaCl, 20mM KH2PO4, 30mM KCl, 0.5% Tween-20, pH 7.4 - Cepham Life Sciences Research Products
![Mobile Phase Buffers in Liquid Chromatography (LC): Effect of Buffer Preparation Method on Retention Repeatability Mobile Phase Buffers in Liquid Chromatography (LC): Effect of Buffer Preparation Method on Retention Repeatability](https://cdn.sanity.io/images/0vv8moc6/chroma/db16ab6672d595100cbb85dc831e9241f426f088-749x465.gif)
Mobile Phase Buffers in Liquid Chromatography (LC): Effect of Buffer Preparation Method on Retention Repeatability
![Calculate pH of a buffer prepared by adding 10 mL of 0.10 M acetic acid to 20 mL of 0.1 M sodium acetate. [pKa (CH3COOH) = 4.74 ] Calculate pH of a buffer prepared by adding 10 mL of 0.10 M acetic acid to 20 mL of 0.1 M sodium acetate. [pKa (CH3COOH) = 4.74 ]](https://i.ytimg.com/vi/t9B5VgPOTG4/maxresdefault.jpg)